Cikakken Bayani
COVID-19 Anti-2020-nCoV Sabon Coronavirus
Kayan gwajin coronavirus COVID-19 na'urar gwajin sauri IgM/IgG gwajin TUV
Novel Coronavirus COVID-19 IgG/IgM Diagnostic Rapid
Marufi & Bayarwa
| Cikakken bayanin marufi: daidaitaccen fakitin fitarwa Bayanin isarwa: a cikin kwanakin aiki 7-10 bayan an karɓi biyan kuɗi |
Ƙayyadaddun bayanai
Novel Coronavirus COVID-19 IgG/IgM Diagnostic Rapid AMRPA69
KA'IDA
Kayan gwajin gaggawa na COVID-19 IgG/IgM (Dukkan Jini/Serum/Plasma) gwaji ne na immunochromatographic na gefe.The
Gwajin yana amfani da anti-human IgM antibody (layin gwaji IgM), anti-human IgG (layin gwajin IgG) da goat anti-zomo IgG (layin sarrafawa C)
immobilized a kan wani nitrocellulose tsiri.Kushin haɗe-haɗe mai launin burgundy ya ƙunshi zinari mai haɗaɗɗiya zuwa
recombinant COVID-19 antigens conjugated da colloid zinariya (COVID-19 conjugates) da zomo IgG-gold conjugates.
Lokacin da samfurin da ke biye da ma'aunin ƙididdiga a cikin samfurin da kyau, IgM &/ko IgG antibodies idan akwai, za su ɗaure zuwa.
COVID-19 yana haɗaka yin hadadden antigen antibodies.Wannan hadaddun yana ƙaura ta hanyar nitrocellulose membrane ta
aikin capillary.Lokacin da hadaddun ya hadu da layin madaidaicin antibody mara motsi (anti-yan adam IgM &/ko
anit-human IgG) hadaddun ya makale yana ƙirƙirar band mai launin burgundy wanda ke tabbatar da sakamakon gwajin aiki.Rashin
band mai launi a yankin gwajin yana nuna sakamakon gwajin da ba ya amsawa.
Gwajin yana ƙunshe da iko na ciki (C band) wanda yakamata ya nuna ƙungiyar burgundy mai launin goat na immunocomplex.
anti zomo IgG/zomo IgG-gold conjugate ko da kuwa da launi ci gaban a kan kowane daga cikin gwajin makada.In ba haka ba, gwajin
Sakamakon ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
HANYAR GWADA
Don samfuran jini ko Plasma:
Bada kayan gwaji, samfuri, buffer da/ko sarrafawa don daidaitawa zuwa zafin jiki (15-30°C) kafin gwaji.
1. Cire tsiri/kaset ɗin gwaji daga jakar jakar da aka rufe kuma a yi amfani da shi da wuri-wuri.Za a sami sakamako mafi kyau idan
Ana yin gwajin a cikin sa'a daya.
2. Sanya kayan gwajin a kan tsaftataccen wuri mai ma'auni.
Strip: Ƙara 2uL na serum/plasma zuwa samfurin samfurin (wuri mai launin ruwan hoda tare da Colloidal zinariya) na gwajin gwajin, sannan ƙara 2 saukad da
(kimanin 60 μL) na samfurin buffer zuwa buffer pad (saman tsiri) nan da nan.
Kaset:
Ƙara 2uL na serum/plasma zuwa samfurin da kyau (A) na kaset ɗin gwajin, sannan ƙara digo 2 (kimanin 60 μL) na samfurin buffer zuwa
buffer da kyau (B) nan da nan.
3. Jira layin (s) masu launi ya bayyana.Ya kamata a karanta sakamakon a minti 10.Ana iya ganin sakamako mai kyau da wuri
kamar minti 2.Kar a fassara sakamakon bayan mintuna 15
