Nā kikoʻī wikiwiki
COVID-19 Anti-2020-nCoV ʻO Coronavirus Hou
pahu hoʻāʻo coronavirus COVID-19 pahu hoʻāʻo wikiwiki IgM/IgG hōʻike TUV
ʻO ka Novel Coronavirus COVID-19 IgG/IgM Diagnostic wikiwiki
Puke a me ka lawe ʻana
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Nā kikoʻī
ʻO ka Novel Coronavirus COVID-19 IgG/IgM Diagnostic Rapid AMRPA69
KUMU
ʻO ka pahu ho'āʻo wikiwiki ʻo COVID-19 IgG/IgM (Whole Blood/Serum/Plasma) kahi hōʻike immunochromatographic kahe ʻaoʻao.ʻO ka
Hoʻohana ka hoʻāʻo i ka anti-human IgM antibody (test line IgM), anti-human IgG (test line IgG) a me kao anti-rabbit IgG (control line C)
immobilized ma ka nitrocellulose strip.Aia i loko o ka papa conjugate kala burgundy ke gula colloidal i hui pū ʻia me
hui hou ʻia nā antigens COVID-19 i hui pū ʻia me ke gula colloid (conjugates COVID-19) a me nā conjugates rabbit IgG-gula.
Ke hoʻohui ʻia kahi mea hoʻohālike i ukali ʻia e ka assay buffer i ka laʻana pūnāwai, IgM &/a i ʻole IgG antibodies inā loaʻa, e hoʻopaʻa ʻia i
ʻO nā conjugates COVID-19 e hana ana i nā antigen antibodies paʻakikī.Ke neʻe nei kēia paʻakikī ma o ka membrane nitrocellulose e
hana capillary.Ke hui ka paʻakikī i ka laina o ka antibody immobilized e pili ana (anti-human IgM &/aiʻole
anit-human IgG) ua paʻa ka paʻakikī i ka hana ʻana i kahi hui kala burgundy e hōʻoia i kahi hopena hoʻokolohua reactive.Ka 'ole o
ʻO kahi pūʻulu kala ma ka ʻāpana hoʻāʻo e hōʻike ana i kahi hopena hoʻāʻo ʻole.
Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ke kao immunocomplex
anti rabbit IgG/rabbit IgG-gold conjugate me ka nānā ʻole i ka hoʻomohala ʻana o ka waihoʻoluʻu ma kekahi o nā kaula hoʻāʻo.A i ʻole, ka hoʻāʻo
ʻAʻole kūpono ka hopena a pono e hoʻāʻo hou ʻia ke ʻano me nā mea hana ʻē aʻe.
HANA HO'O
No ka Serum a i ʻole Plasma Specimens:
E ʻae i ka pahu hoʻāʻo, specimen, buffer a/a i ʻole nā mana e hoʻohālikelike i ka mahana lumi (15-30°C) ma mua o ka hoʻāʻo ʻana.
1. Wehe i ka pahu ho'āʻo / cassette mai ka ʻeke pepa i hoʻopaʻa ʻia a hoʻohana koke i ka hiki.E loaʻa nā hopena maikaʻi loa inā
hana ʻia ka hoʻāʻo ʻana i loko o hoʻokahi hola.
2. E kau i ka pahu ho'āʻo ma kahi maʻemaʻe a palahalaha.
Strip: E hoʻohui i 2uL o ka serum/plasma i ka papa hoʻohālike (wahi poni me ke gula Colloidal) o ka ʻili hoʻāʻo, a laila hoʻohui i 2 kulu.
(e pili ana i 60 μL) o ka laʻana pale i ka papa pale (luna o ke kaula) koke.
Kapepa:
E hoʻohui i 2uL o ka serum/plasma i ka pūnāwai specimen(A) o ka pahu hoʻāʻo, a laila e hoʻohui i 2 kulu (ma kahi o 60 μL) o ka laʻana paʻa i
ka punawai pale (B) koke.
3. E kali a puka mai ka laina kala.Pono e heluhelu ʻia ka hopena ma 10 mau minuke.Hiki ke ʻike koke ʻia nā hopena maikaʻi
e like me 2 minuke.Mai wehewehe i ka hopena ma hope o 15 mau minuke
