Akwụkwọ akụkọ Coronavirus COVID-19 IgG/IgM Diagnostic Rapid

Nkọwa ngwa ngwa

COVID-19 Mgbochi-2020-nCoV Coronavirus ọhụrụ
ngwa nyocha coronavirus COVID-19 ngwa ule ngwa ngwa IgM/IgG test TUV
Akwụkwọ akụkọ Coronavirus COVID-19 IgG/IgM Diagnostic Rapid

Nkwakọ ngwaahịa & nnyefe

Nkọwapụta

AMRPA68
Ngwa nyocha ngwa ngwa COVID-19 IgM/IgG
(Immunochromatography)
Aha ngwaahịa
Ngwa nyocha ngwa ngwa COVID-19 IgM/IgG
(Immunochromatography)
Ezubere iji
A na-eji reagent chọpụta nje Corona-19 IgM/IgG Antibody in
serum/plasma/ọbara dum nke ọma.
UWU Nnwale
Ngwa a dabere na ụkpụrụ nke akara ọla edo immunochromatographic nnwale ma na-eji usoro njide iji chọpụta ihe mgbochi COVID-19 IgM/IgG dị na nlele ahụ.

UWU Nnwale

COVID-19 IgM
Mgbe ihe nlele ahụ nwere mgbochi COVID-19 IgM, ọ na-eme mgbagwoju anya nwere akara antigen (COVID-19 recombinant antigen).Ihe mgbagwoju anya na-aga n'ihu n'okpuru ọrụ nke chromatography ma jikọta ya na mgbochi mkpuchi (Mouse anti-human IgM monoclonal antibody) na T akara iji mepụta mgbagwoju anya na ịmepụta agba (T line), nke bụ ihe dị mma.Mgbe ihe nlele ahụ enweghị ihe mgbochi COVID-19 IgM, enweghị mgbagwoju anya enwere ike ịmepụta n'ahịrị T, ọ nweghị eriri uhie pụtara, nke bụ nsonaazụ na-adịghị mma.
N'agbanyeghị ma mgbochi COVID-19 IgM dị n'ime ihe nlele ahụ, akara njikwa akara ọla edo (oke bekee IgG antibody) ga-ejikọta ya na mgbochi mkpuchi mkpuchi (ewu mgbochi oke bekee IgG) na ahịrị C iji mepụta mgbagwoju anya ma tolite. agba (akara C).

COVID-19 IgG
Mgbe ihe nlele ahụ nwere ihe mgbochi COVID-19 IgG, ọ na-eme mgbagwoju anya nwere akara antigen (COVID-19 recombinant antigen).Ihe mgbagwoju anya na-aga n'ihu n'okpuru omume nke chromatography ma jikọta ya na mgbochi mkpuchi (Mouse anti-human IgG monoclonal antibody) na T akara iji mepụta mgbagwoju anya na ịmepụta agba (T line), nke bụ ihe dị mma.Mgbe ihe nlele ahụ enweghị ihe mgbochi COVID-19 IgG, enweghị ike ịmepụta n'ahịrị T, ọ nweghị eriri uhie pụtara, nke bụ nsonaazụ na-adịghị mma.
 

N'agbanyeghị ma mgbochi COVID-19 IgG dị n'ime ihe nlele ahụ, akara njikwa akara ọla edo (oke bekee IgM antibody) ga-ejikọta ya na mgbochi mkpuchi mkpuchi (ewu mgbochi oke bekee IgG) na ahịrị C iji mepụta mgbagwoju anya ma tolite. agba (akara C).

Ihe ndị bụ isi
COVID-19 IgM: T-line kpuchie na òké mgbochi mmadụ IgM monoclonal antibody, ọla edo akara pad siri ike COVID-19 antigen recombinant, oke bekee IgG mgbochi, C-akara mkpuchi ewu mgbochi oke bekee IgG mgbochi.

COVID-19 IgG: T-ahịrị kpuchie na òké mgbochi mmadụ IgG monoclonal mgbochi, ọla edo akara pad siri ike COVID-19 antigen recombinant, oke bekee IgM mgbochi, C-akara kpuchie na ewu mgbochi oke bekee IgM mgbochi.Nlere anya dilution: nke 20 mM phosphate buffer solution (PBS) mejupụtara.

Nchekwa na EXPIRY
Chekwaa dị ka ngwugwu a na-emechi emechi na 4-30 ℃, zere ikpo ọkụ na anwụ, ebe akọrọ, dị irè maka ọnwa 12.Emela friji.Ekwesịrị ịme ụfọdụ usoro nchebe n'oge okpomọkụ na oyi oyi iji zere oke okpomọkụ ma ọ bụ kpochapu.Emeghela nkwakọ ngwaahịa dị n'ime ruo mgbe ọ dị njikere, a ga-eji ya mee ihe n'otu awa ma ọ bụrụ na emeghere (Iru mmiri≤60%, Temp: 20℃-30℃).Biko jiri ozugbo mgbe iru mmiri ruru 60%.

Nlereanya chọrọ
1. Enwere ike iji reagent maka ọbara ọbara, plasma na nlele ọbara dum.
2. A ga-anakọta ihe nlele ọbara ọbara / serum / plasma n'ime akpa dị ọcha ma kpọọ nkụ.Enwere ike iji EDTA, sodium citrate, heparin dị ka anticoagulants na plasma / n'ọbara ọbara dum.Chọpụta ozugbo ị nwetachara ọbara.
3.Serum na plasma samples nwere ike ịchekwa na 2-8 ℃ maka 3 ụbọchị tupu assay.Ọ bụrụ na-egbu oge ule karịa ụbọchị 3, ihe nlele ahụ kwesịrị ka oyi kpọnwụrụ (-20 ℃ ma ọ bụ oyi).Tinyegharịa friji wee mee ka ọ dịkarịa ala ugboro atọ.Enwere ike ịchekwa ihe nlele ọbara zuru oke nke nwere ọgwụ mgbochi obi na 2-8 ℃ maka ụbọchị 3, ma ekwesịghị ka oyi kpọnwụrụ;Ekwesịrị iji ụdị ọbara niile na-enweghị ọgwụ mgbochi mkpali ozugbo (ọ bụrụ na nlele ahụ nwere agglutination, enwere ike ịchọpụta ya site na serum).

Uzo esi anwale
A ga-agụrịrị ntuziaka tupu emee ule ahụ.Kwe ka njikwa ngwaọrụ nwale kwekọọ na ọnụ ụlọ maka nkeji 30 (20 ℃-30 ℃) tupu nnwale.Emeghela nkwakọ ngwaahịa dị n'ime ruo mgbe ọ dị njikere, a ga-eji ya mee ihe n'otu awa ma ọ bụrụ na emeghere (Iru mmiri≤60%, Temp: 20℃-30℃).Biko jiri ozugbo mgbe iru mmiri ruru 60%.
Maka Serum/Plasma
1. Wepu ngwaọrụ nyocha site na obere akpa ahụ mechiri emechi, tinye ya n'elu ebe dị ọcha ma dị larịị na ihe nlele ahụ dị elu.
2. Tinye otu (1) zuru oke nke serum ma ọ bụ plasma (10μl) kwụ ọtọ n'ime olulu mmiri nke IgM na IgG iche.
3. Tinye abụọ (2) tụlee (80-100μl) nke ihe nchekwa ihe n'ime ihe nlele nke IgM na IgG iche.
4. Lelee nsonaazụ ule ozugbo n'ime nkeji 15 ~ 20, nsonaazụ ya adịghị mma karịa nkeji 20.

COVID-19 IgG
Nyocha nke ọnụọgụ ndaba nke COVID-19 IgG Ab ule ngwa ngwa na nucleic acid reagent na nlele serum:
Ọnụego ndakọrịta dị mma = 46 / (46+4) × 100% = 92%,
Ọnụọgụgụ ndaba na-adịghị mma=291 / (9+291) × 100% = 97%,
Ngụkọta ọnụ ọgụgụ ndabanyere =(46+291) / (46+4+9+291) × 100% = 96.3%.

COVID-19 IgM
Nyocha nke ọnụọgụ ndaba nke COVID-19 IgM Ab ule ngwa ngwa na acid nucleic
reagent na serum sample:
Ọnụego ndakọrịta dị mma = 41 / (41+9) × 100% = 82%,
Ọnụọgụ ndaba na-adịghị mma=282 / (18+282) × 100% = 94%,
Mkpokọta ọnụ ọgụgụ ndaba =(41+282) / (41+9+18+282) × 100% = 92.3%

Nlebara anya
1. N'ihi na IN VITRO diagnostic were naanị.
2. Reagents kwesịrị iji ngwa ngwa o kwere mee mgbe emepere ya.Enweghị ike ijikwa reagent a maka mkpofu.
3. Ngwa ule kwesịrị ịdị na obere akpa akara ruo mgbe ejiri ya.Ọ bụrụ na nsogbu akara emee, nwalela.Ejila mgbe ụbọchị njedebe gachara.
4.All specimens na reagents kwesịrị-atụle nwere ike dị ize ndụ na edozi n'otu ụzọ ahụ dị ka ihe na-efe efe gị n'ụlọnga mgbe ojiji.