Nga korero tere
COVID-19 Anti- 2020-nCoV Coronavirus Hou
kete whakamatautau coronavirus COVID-19 kete whakamatautau tere IgM/IgG whakamātautau TUV
Ko te Novel Coronavirus COVID-19 IgG/IgM Tere Tatau
Te Whakapaipai me te Tukunga
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Whakatakotoranga
AMRPA68
COVID-19 IgM/IgG Antibody Rapid Test Kit
(Immunochromatography)
Ingoa HUA
COVID-19 IgM/IgG Antibody Rapid Test Kit
(Immunochromatography)
WHAKAMAHI WHAKAMAHI
Ka whakamahia te reagent ki te kite i te Corona Virus-19 IgM/IgG Antibody i roto
serum/plasma/toto katoa qualitatively.
MĀTĀPONO WHAKAMATAU
I ahu mai tenei kete i runga i te maapono o te whakamatautau immunochromatographic tapanga koura me te whakamahi i nga tikanga hopu kia kitea ai te COVID-19 IgM/IgG antibody i roto i te tauira.
MĀTĀPONO WHAKAMATAU
COVID-19 IgM
I te wa e mau ana te tauira i te COVID-19 IgM antibody, ka hanga he matatini me te antigen tapanga koura (COVID-19 recombinant antigen).Ka neke whakamua te matatini i raro i te mahi o te chromatography me te whakakotahi ki te antibody pani (Mouse anti-human IgM monoclonal antibody) i te raina T ki te hanga i te matatini me te whakawhanake i te tae (raina T), he hua pai.Ki te kore te tauira i roto i te COVID-19 IgM antibody, karekau he matatini e taea te hanga i te raina T, karekau he roopu whero ka puta, he hua kino.
Ahakoa kei roto te COVID-19 IgM antibody i roto i te tauira, ka herea te antibody mana kounga tapanga koura (rabbit IgG antibody) ki te antibody pani (koati anti-rabbit IgG antibody) i te raina C ki te hanga i te matatini me te whakawhanake. tae (raina C).
COVID-19 IgG
Ina kei roto i te tauira te antibody COVID-19 IgG, ka hanga he matatini me te antigen tapanga koura (COVID-19 recombinant antigen).Ka neke whakamua te matatini i raro i te mahi o te chromatography me te whakakotahi ki te antibody pani (Mouse anti-human IgG monoclonal antibody) i te raina T ki te hanga i te matatini me te whakawhanake i te tae (raina T), he hua pai.Ki te kore te tauira i roto i te COVID-19 IgG antibody, karekau he matatini e taea te hanga i te raina T, karekau he roopu whero ka puta, he hua kino.
Ahakoa kei roto te COVID-19 IgG antibody i roto i te tauira, ka herea te antibody mana o te kounga tapanga koura (rabbit IgM antibody) ki te antibody pani (koati anti-rabbit IgG antibody) i te raina C ki te hanga i tetahi matatini me te whakawhanake. tae (raina C).
NGA WHARE MATUA
COVID-19 IgM: I whakakikoruatia te raina-T ki te kiore anti-tangata IgM monoclonal antibody, papa tapanga koura wahanga totoka COVID-19 recombinant antigen, rabbit IgG antibody, C-line kua pania ki te koati anti-rabbit IgG antibody.
COVID-19 IgG: I whakakikoruatia te raina-T ki te kiore anti-tangata IgG monoclonal antibody, papa tapanga koura wahanga totoka COVID-19 recombinant antigen, rapeti IgM antibody, C-line kua pania ki te koati anti-rabbit IgM antibody.Tauira waimehatanga: 20 mM phosphate buffer solution (PBS)
TE WHAKAPUTANGA ME TE MUTU
Whakaorangia i roto i te putea hiri i te 4-30 ℃, karohia te wera me te ra, te wahi maroke, whai mana mo te 12 marama.KAUA E WHAKAMATE.Ko etahi tikanga whakamarumaru me mahi i te raumati wera me te takurua makariri kia kore ai te werawera teitei, te tio rewa ranei.Kaua e whakatuwheratia te kohinga o roto kia rite ra ano, me whakamahi i roto i te haora kotahi ki te whakatuwherahia (Humidity≤60%, Temp: 20℃-30℃).Me whakamahi tonu ina te haumākū> 60%.
HE WHAKAMAHI TAUIRA
1. Ka taea te whakamahi i te reagent mo te serum, plasma me nga tauira toto katoa.
2. Me kohia he tauira serum / plasma / toto katoa ki roto i te ipu ma, maroke.EDTA, konutai citrate, heparin ka taea te whakamahi hei anticoagulants i roto i nga tauira plasma / toto katoa.Rapua i muri tonu i te kohi toto.
Ka taea te rongoa 3.Serum me nga tauira plasma ki te 2-8 ℃ mo nga ra 3 i mua i te whakamatautau.Mena ka roa ake te whakamatautau i te 3 ra, me whakatio te tauira (-20 ℃, makariri ake ranei).Tukuruatia te whakatio me te rewa kia kaua e neke ake i te 3 nga wa.Ko nga tauira toto katoa me te anticoagulant ka taea te rongoa ki te 2-8 ℃ mo te 3 ra, kaua hoki e whakatotoka;Ko nga tauira toto katoa kaore he anticoagulant me whakamahi tonu (mehemea he agglutination te tauira, ka kitea e te serum).
NGA WHAKAMAHI
Me panui katoa nga tohutohu i mua i te tango i te whakamatautau.Whakaaetia te mana o te taputapu whakamatautau kia rite ki te pāmahana rūma mo te 30 meneti (20 ℃-30 ℃) i mua i te whakamatautau.Kaua e whakatuwheratia te kohinga o roto kia rite ra ano, me whakamahi i roto i te haora kotahi ki te whakatuwherahia (Humidity≤60%, Temp: 20℃-30℃).Me whakamahi tonu ina te haumākū> 60%.
Mo te Serum / Plasma
1. Tangohia te taputapu whakamatautau mai i te putea hiri, tuu ki runga i te mata ma me te papatahi me te pai o te tauira.
2. Tāpirihia kia kotahi (1) te topata katoa o te serum, plasma ranei (10μl) poutū ki roto i te puna tauira o IgM me IgG motuhake.
3. Tāpirihia kia rua (2) pata (80-100μl) o te tauira parepare ki roto i te puna tauira o IgM me IgG motuhake.
4. Tirohia nga hua whakamatautau i roto i nga meneti 15 ~ 20, he muhu te hua mo te 20 meneti
COVID-19 IgG
Te tātaritanga o te reeti ohorere o te COVID-19 IgG Ab te whakamatautau tere me te reagent waikawa nucleic i roto i nga tauira serum:
Reeti coincidence pai=46 / (46+4) × 100% = 92%,
Reeti coincidence tōraro=291 / (9+291) × 100% = 97%,
Te tapeke o te reanga tupono=(46+291) / (46+4+9+291) × 100% = 96.3%.
COVID-19 IgM
Te tātari i te reeti ohorere o te COVID-19 IgM Ab whakamātautau tere me te waikawa karihi
reagent i roto i nga tauira serum:
Reeti coincidence pai=41 / (41+9) × 100% = 82%,
Reeti coincidence tōraro=282 / (18+282) × 100% = 94%,
Te tapeke o te reeti ohorere=(41+282) / (41+9+18+282) × 100% = 92.3%
WHAKATOKANGA
1. Mo te whakamahi tātaritanga IN VITRO anake.
2. Me whakamahi wawe nga reagents i muri i te whakatuwheratanga.Kaore e taea te whakamahi ano i tenei reagent mo te tuku.
3. Me noho tonu te taputapu whakamatautau ki roto i nga putea hiri kia whakamahia ra ano.Mena ka pa mai te raru o te hiri, kaua e whakamatautau.Kaua e whakamahi i muri i te ra paunga.
4. Ko nga tauira me nga reagents katoa me whakaaro he mea morearea, ka whakahaeretia i runga ano i te ahua o te kaihoko hopuhopu i muri i te whakamahinga.
