Nga korero tere
COVID-19 Anti- 2020-nCoV Coronavirus Hou
kete whakamatautau coronavirus COVID-19 kete whakamatautau tere IgM/IgG whakamātautau TUV
Ko te Novel Coronavirus COVID-19 IgG/IgM Tere Tatau
Te Whakapaipai me te Tukunga
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Whakatakotoranga
Novel Coronavirus COVID-19 IgG/IgM Tātari Tere AMRPA69
MĀTĀPONO
Ko te kete COVID-19 IgG/IgM Rapid Test kit (Toko Katoa/Serum/Plasma) he whakamatautau immunochromatographic rere taha.Ko te
Ka whakamahia e te whakamatautau te anti-tangata IgM antibody (whakamatautau IgM), anti-tangata IgG (whakamatautau raina IgG) me te koati anti-rabbit IgG (raina mana C)
kare i nekehia i runga i te riu nitrocellulose.Kei roto i te papa conjugate tae burgundy he koura colloidal kua honoa ki
ko nga antigens COVID-19 kua honoa ki te koura colloid (ko te COVID-19 conjugates) me te rapeti IgG-koura conjugates.
I te wa ka taapirihia he tauira i muri mai i te parepare whakamatautau ki te pai o te tauira, ka herea te IgM &/ranei IgG antibody mena kei reira.
Ko nga hononga COVID-19 e hanga ana i nga antigen antibodies matatini.Ka heke tenei matatini na roto i te membrane nitrocellulose na
mahi capillary.Ina tutaki te matatini ki te raina o te antibody immobilized e rite ana (IgM anti-tangata &/ranei
anit-human IgG) ka mau te matatini ki te hanga i tetahi roopu tae burgundy e whakau ana i te hua whakamatautau.Te kore o
ko te roopu tae i te rohe whakamatautau e tohu ana he hua whakamatautau kore-hohe.
Kei roto i te whakamatautau he mana-a-roto (C band) me whakaatu he roopu tae burgundy o te koati immunocomplex
anti rabbit IgG/rabbit IgG-koura conjugate ahakoa te whanaketanga o te tae ki tetahi o nga roopu whakamatautau.Ki te kore, ko te whakamatautau
he muhu te hua, me whakamatau ano te tauira ki tetahi atu taputapu.
TE WHAKAMAHI WHAKAMAHI
Mo nga Tauira Serum, Plasma ranei:
Whakaaetia te kete whakamatautau, tauira, parepare me/ranei nga mana whakahaere kia taurite ki te pāmahana rūma (15-30°C) i mua i te whakamatautau.
1. Tangohia te riipene whakamatautau mai i te putea konumohe kua hirihia ka whakamahia wawetia.Ka whiwhi hua pai mena
ka mahia te whakamatautau i roto i te haora kotahi.
2. Whakanohoia te kete whakamatautau ki runga i te mata ma me te taumata.
Wewetehia: Taapirihia te 2uL o te serum/plasma ki te papa tauira (wahi papura me te Colloidal koura) o te riipene whakamatautau, katahi ka taapiri kia 2 nga pata.
(tata ki te 60 μL) o te tauira parepare ki te papa papare (runga o te takai) tonu.
Rīpene:
Tāpirihia te 2uL o te serum/plasma ki te puna tauira(A) o te rīpene whakamātautau, kātahi ka tāpirihia kia 2 pata (tata ki te 60 μL) o te tauira parepare ki
te pai o te kaitapa (B) tonu.
3. Tatari kia puta mai nga rarangi tae.Me panui te hua i te 10 meneti.Ka kitea pea nga hua pai
hei 2 meneti.Kaua e whakamaoritia te hua i muri i te 15 meneti
