Novel Coronavirus COVID-19 IgG/IgM Diagnostic Rapid

Zambiri Zachangu

COVID-19 Anti-2020-nCoV Coronavirus Watsopano
zida zoyeserera za coronavirus COVID-19 zida zoyeserera mwachangu za IgM/IgG TUV
Novel Coronavirus COVID-19 IgG/IgM Diagnostic Rapid

Kupaka & Kutumiza

Zofotokozera

AMRPA68
COVID-19 IgM/IgG Antibody Rapid Test Kit
(Immunochromatography)
PRODUCT NAME
COVID-19 IgM/IgG Antibody Rapid Test Kit
(Immunochromatography)
ZOYENERA KUGWIRITSA NTCHITO
The reagent imagwiritsidwa ntchito kuzindikira kachilombo ka Corona Virus-19 IgM/IgG Antibody mkati
seramu/plasma/magazi athunthu moyenerera.
MFUNDO YOYESA
Zidazi zimatengera mfundo ya kuyesa kwa golide label immunochromatographic ndipo imagwiritsa ntchito njira yojambulira kuti izindikire kachilombo ka COVID-19 IgM/IgG mu zitsanzo.

MFUNDO YOYESA

COVID-19 IgM
Zitsanzozi zikakhala ndi anti-COVID-19 IgM, zimakhala zovuta kukhala ndi antigen yagolide (COVID-19 recombinant antigen).Zovutazo zimapita patsogolo pansi pa machitidwe a chromatography ndikuphatikiza ndi antibody yokutira (Mouse anti-human IgM monoclonal antibody) pamzere wa T kuti apange mtundu wovuta ndikukulitsa (T mzere), zomwe ndi zotsatira zabwino.Ngati chitsanzocho chilibe anti-COVID-19 IgM, palibe zovuta zomwe zingapangidwe pamzere wa T, ndipo palibe gulu lofiira lomwe likuwoneka, zomwe ndi zotsatira zoyipa.
Mosasamala kanthu kuti anti-COVID-19 IgM antibody ili mu chitsanzocho, antibody ya golide (antibody ya kalulu IgG) imamanga ndi anti-anti-kalulu IgG antibody) pamzere wa C kuti ipange zovuta ndikupanga mtundu (C mzere).

COVID-19 IgG
Chitsanzocho chikakhala ndi anti-antibody ya COVID-19 IgG, chimapanga chovuta chokhala ndi antigen yagolide (COVID-19 recombinant antigen).Zovutazo zimapita patsogolo pansi pa zochitika za chromatography ndikuphatikiza ndi antibody yokutira (Mouse anti-human IgG monoclonal antibody) pamzere wa T kuti apange mtundu wovuta ndikukulitsa (T mzere), zomwe ndi zotsatira zabwino.Ngati chitsanzocho chilibe anti-COVID-19 IgG, palibe zovuta zomwe zingapangidwe pamzere wa T, ndipo palibe gulu lofiira lomwe likuwoneka, zomwe ndi zotsatira zoyipa.
 

Mosasamala kanthu kuti anti-COVID-19 IgG antibody ili m'chitsanzocho, antibody yowongolera khalidwe la golide (antibody ya kalulu IgM) imamanga ndi anti-anti-kalulu IgG antibody) pamzere wa C kuti ipange zovuta ndikupanga mtundu (C mzere).

ZINTHU ZOFUNIKA KWAMBIRI
COVID-19 IgM: T-line yokutidwa ndi mbewa anti-anthu IgM monoclonal antibody, golide label pad solid phase COVID-19 recombinant antigen, kalulu IgG antibody, C-line yokutidwa ndi mbuzi anti-kalulu IgG antibody.

COVID-19 IgG: T-line yokutidwa ndi mbewa anti-anthu IgG monoclonal antibody, golide label pad solid phase COVID-19 recombinant antigen, kalulu IgM antibody, C-line yokutidwa ndi mbuzi anti-kalulu IgM antibody.Kusungunuka kwachitsanzo: wopangidwa ndi 20 mM phosphate buffer solution (PBS)

KUSINTHA NDI KUTHA
Sungani monga zopakidwa muthumba losindikizidwa pa 4-30 ℃, pewani kutentha ndi dzuwa, malo owuma, ovomerezeka kwa miyezi 12.OSATI MASIMA.Njira zina zodzitetezera ziyenera kuchitidwa m'chilimwe komanso m'nyengo yozizira kuti musatenthe kwambiri kapena kuzizira.Osatsegula ma CD amkati mpaka atakonzeka, ayenera kugwiritsidwa ntchito mu ola limodzi ngati atatsegulidwa (Chinyezi≤60%, Temp: 20℃-30 ℃).Chonde ntchito yomweyo pamene chinyezi>60%.

ZOFUNIKA ZITSANZO
1. The reagent ingagwiritsidwe ntchito pa seramu, plasma ndi magazi athunthu.
2. Seramu / plasma / magazi athunthu atengedwe mu chidebe choyera ndi chowuma.EDTA, sodium citrate, heparin angagwiritsidwe ntchito ngati anticoagulants mu plasma / lonse magazi zitsanzo.Dziwani mwamsanga mutatolera magazi.
Zitsanzo za 3.Seramu ndi plasma zitha kusungidwa ku 2-8 ℃ kwa masiku atatu musanayesedwe.Ngati kuyezetsa kuchedwa kupitilira masiku atatu, chitsanzocho chiyenera kuzizira (-20 ℃ kapena kuzizira).Bwerezani kuzizira ndi kusungunuka kwa nthawi zosapitirira 3.Zitsanzo zamagazi athunthu okhala ndi anticoagulant zitha kusungidwa pa 2-8 ℃ kwa masiku atatu, ndipo sayenera kuzizira;Zitsanzo zonse za magazi popanda anticoagulant ziyenera kugwiritsidwa ntchito nthawi yomweyo (ngati chitsanzocho chili ndi agglutination, chikhoza kudziwika ndi seramu).

NJIRA ZOYESA
Malangizo ayenera kuwerengedwa kwathunthu musanayesedwe.Lolani zowongolera zida zoyeserera kuti zigwirizane ndi kutentha kwachipinda kwa mphindi 30 (20 ℃-30 ℃) zisanachitike.Osatsegula ma CD amkati mpaka atakonzeka, ayenera kugwiritsidwa ntchito mu ola limodzi ngati atatsegulidwa (Chinyezi≤60%, Temp: 20℃-30 ℃).Chonde ntchito yomweyo pamene chinyezi>60%.
Kwa Serum / Plasma
1. Chotsani chipangizo choyesera m'thumba lomatapo, chiyikeni pamalo oyera komanso osalala bwino ndipo chitsanzocho chili bwino.
2. Onjezani dontho limodzi (1) lathunthu la seramu kapena plasma (10μl) molunjika mu chitsime cha IgM ndi IgG padera.
3. Onjezani madontho awiri (2) (80-100μl) a bafa yachitsanzo mu chitsime cha IgM ndi IgG padera.
4. Yang'anani zotsatira za mayeso nthawi yomweyo mkati mwa mphindi 15-20, zotsatira zake zimakhala zosavomerezeka pa mphindi 20

COVID-19 IgG
Kuwunika kwa kuphatikizika kwa COVID-19 IgG Ab kuyesa mwachangu ndi nucleic acid reagent mu zitsanzo za seramu:
Mlingo wabwino wangochitika mwangozi = 46 / (46+4) × 100% = 92%,
Mlingo wolakwika = 291 / (9+291) × 100% = 97%,
Chiwerengero chonse changochitika mwangozi = (46+291) / (46+4+9+291) × 100% = 96.3%.

COVID-19 IgM
Kuwunika kwa kuphatikizika kwa COVID-19 IgM Ab kuyesa mwachangu ndi nucleic acid
reagent mu seramu zitsanzo:
Mlingo wabwino wangochitika mwangozi = 41 / (41 + 9) × 100% = 82%,
Mlingo wolakwika = 282 / (18+282) × 100% = 94%,
Chiwerengero chonse changochitika mwangozi =(41+282) / (41+9+18+282) × 100% = 92.3%

CHENJEZO
1. Pakuti IN VITRO diagnostic ntchito kokha.
2. Ma reagents ayenera kugwiritsidwa ntchito posachedwa atatsegulidwa.Izi reagent sangathe kugwiritsidwanso ntchito kuti disposable.
3. Chipangizo choyesera chizikhala m'matumba osindikizidwa mpaka chigwiritsidwe ntchito.Ngati vuto losindikiza lichitika, musayese.Osagwiritsa ntchito tsiku lotha ntchito litatha.
4.Zitsanzo zonse ndi ma reagents ziyenera kuganiziridwa kuti zingakhale zoopsa ndipo zimayendetsedwa mofanana ndi wothandizira opatsirana pambuyo pogwiritsira ntchito.