Ibisobanuro Byihuse
Suzuma ahari Anaplasma spp
Suzuma Igihe: iminota 5-10
Icyitegererezo: Serumu, plasma
Gupakira & Gutanga
Ibipapuro birambuye: Ibicuruzwa byoherezwa hanze Ibisobanuro birambuye: muminsi 7-10 y'akazi nyuma yo kubona ubwishyu |
Ibisobanuro
Ikiboneka cyihuta Ikizamini Cassette AMDH47B
UKORESHEJWE
Ikizamini kitagaragara cyihuta Cassette AMDH47B ni cassette yikizamini kugirango isuzume ahari Anaplasma spp.antibodies muri serumu yimbwa.
Suzuma Igihe: iminota 5-10
Icyitegererezo: Serumu, plasma.
IHame
Ikizamini kitagaragara cyihuta Cassette AMDH47B ishingiye kuri sandwich kuruhande rwa immunochromatographic assay.Ikarita yikizamini ifite idirishya ryikizamini cyo kwitegereza ibizamini bikora no gusoma ibisubizo.Idirishya ryikizamini rifite zone T (ikizamini) itagaragara na zone C (igenzura) mbere yo gukora assay.
Iyo icyitegererezo kivuwe cyashyizwe mu mwobo w'icyitegererezo ku gikoresho, amazi azahita atembera hejuru y’ikizamini hanyuma akore hamwe na antigens ya recombinant ya Anaplasma yabanje gushyirwaho.Niba hari antibodiyite za Anaplasma murugero, umurongo T ugaragara uzagaragara.Umurongo C ugomba guhora ugaragara nyuma yicyitegererezo cyakoreshejwe, cyerekana ibisubizo byemewe.Ukoresheje ubu buryo, igikoresho gishobora kwerekana neza ko hariho antibodiyite za Anaplasma murugero.
Ikiboneka cyihuta Ikizamini Cassette AMDH47B
REAGENTS N'IBIKORWA
- Ibikoresho byo kugerageza, hamwe nibitonyanga
- Suzuma buffer
- Igitabo gikubiyemo ibicuruzwa
Ububiko N'UBUHAMYA
Ibikoresho birashobora kubikwa mubushyuhe bwicyumba (4-30 ° C).Ikizamini cyikizamini gihamye binyuze mumatariki yo kurangiriraho yashyizwe kumurongo.NTUBUNTU.Ntukabike ibikoresho byo kwipimisha ku zuba.
GUTEGURA BYIHARIYE N'UBubiko
1. Ibigereranyo bigomba kuboneka no gufatwa nkibi bikurikira.
- Serumu cyangwa plasma: gukusanya amaraso yose kuminjangwe yumurwayi, centrifuge kugirango ibone plasma, cyangwa shyira amaraso yose mumiyoboro irimo anticagulants kugirango ubone serumu.
- Amazi ya Pleural cyangwa asitike: gukusanya amazi ya pleural fluid cyangwa asitike yimbwa yumurwayi.Koresha mu buryo butaziguye muri assay cyangwa kubika kuri 2-8 ℃.
2. Ingero zose zigomba guhita zipimwa.Niba atari ukugerageza nonaha, bigomba kubikwa kuri 2-8 ℃.