Ibishya Coronavirus COVID-19 IgG / IgM Gusuzuma Byihuse

Ibisobanuro Byihuse

COVID-19 Anti-2020-nCoV Coronavirus Nshya
ibikoresho byo gupima coronavirus COVID-19 igeragezwa ryihuse IgM / IgG ikizamini TUV
Ibishya Coronavirus COVID-19 IgG / IgM Gusuzuma Byihuse

Gupakira & Gutanga

Ibisobanuro

AMRPA68
COVID-19 IgM / IgG Antibody Yihuta Ikizamini
(Immunochromatography)
IZINA RY'IGICURUZWA
COVID-19 IgM / IgG Antibody Yihuta Ikizamini
(Immunochromatography)
UKORESHEJWE
Reagent ikoreshwa mugutahura virusi ya Corona-19 IgM / IgG Antibody muri
serumu / plasma / amaraso yose yujuje ubuziranenge.
IHAME RY'IKIZAMINI
Iki gikoresho gishingiye ku ihame rya label label ya immunochromatographic kandi ikoresha uburyo bwo gufata kugirango umenye antibody ya COVID-19 IgM / IgG muri sample.

IHAME RY'IKIZAMINI

COVID-19 IgM
Iyo icyitegererezo kirimo antibody ya COVID-19 IgM, ikora urwego rugizwe na label ya zahabu antigen (COVID-19 recombinant antigen).Urusobekerane rugenda rutera imbere munsi ya chromatografiya kandi igahuza na antibody yubatswe (Mouse anti-human IgM monoclonal antibody) kumurongo wa T kugirango igire urwego rugoye kandi rutezimbere ibara (T umurongo), nigisubizo cyiza.Iyo icyitegererezo kitarimo antibody ya COVID-19 IgM, nta complexe ishobora gushingwa kumurongo wa T, kandi nta bande itukura igaragara, nigisubizo kibi.
Hatitawe ku kumenya niba antibody ya COVID-19 IgM ikubiye muri sample, label ya zahabu irwanya antibody (urukwavu IgG antibody) izahuza na antibody yometseho (antibody y'ihene irwanya urukwavu IgG) kumurongo wa C kugirango ibe igoye kandi itere imbere ibara (C umurongo).

COVID-19 IgG
Iyo icyitegererezo kirimo antibody ya COVID-19 IgG, ikora urwego rufite ikirango cya zahabu antigen (COVID-19 recombinant antigen).Urusobekerane rugenda rutera imbere munsi ya chromatografiya kandi igahuza na antibody yubatswe (Mouse anti-human IgG monoclonal antibody) kumurongo wa T kugirango ibe igoye kandi itezimbere ibara (T umurongo), nigisubizo cyiza.Iyo icyitegererezo kitarimo antibody ya COVID-19 IgG, nta complexe ishobora gushingwa kumurongo wa T, kandi nta bande itukura igaragara, nigisubizo kibi.
 

Hatitawe ku kumenya niba antibody ya COVID-19 IgG ikubiye mu cyitegererezo, antibody ya label yo kugenzura ubuziranenge bwa antibody (urukwavu IgM antibody) izahuza na antibody isize (ihene irwanya urukwavu IgG antibody) kumurongo wa C kugirango ibe igoye kandi itere imbere ibara (C umurongo).

INGINGO Z'INGENZI
COVID-19 IgM: T-umurongo washyizweho na antibody irwanya muntu IgM monoclonal antibody, zahabu label pad ikomeye icyiciro cya COVID-19 recombinant antigen, urukwavu IgG antibody, C-umurongo washyizwemo na ihene irwanya urukwavu IgG antibody.

COVID-19 IgG: T-umurongo washyizweho na antibody irwanya abantu IgG monoclonal antibody, zahabu label pad icyiciro gikomeye COVID-19 antombine antigen, urukwavu IgM antibody, C-umurongo wasize ihene irwanya urukwavu IgM antibody.Icyitegererezo cyogusohora: kigizwe na mM 20 ya fosiferi yo gukemura (PBS)

KUBAKA KANDI BISANZWE
Ubike nkuko bipakiye mumufuka ufunze kuri 4-30 ℃, irinde ubushyuhe nizuba, ahantu humye, byemewe mumezi 12.NTUBUNTU.Hagomba gufatwa ingamba zo gukingira mu gihe cyizuba nubukonje bukabije kugirango wirinde ubushyuhe bwinshi cyangwa ubukonje bukabije.Ntukingure ipaki yimbere kugeza yiteguye, igomba gukoreshwa mumasaha imwe iyo ifunguye (Ubushuhe≤60%, Ubushyuhe: 20 ℃ -30 ℃).Nyamuneka koresha ako kanya mugihe ubuhehere ＞ 60%.

ICYITONDERWA
1. Reagent irashobora gukoreshwa kuri serumu, plasma hamwe namaraso yose.
2. Serumu / plasma / icyitegererezo cyamaraso kigomba gukusanyirizwa mubintu bisukuye kandi byumye.EDTA, citrate ya sodium, heparin irashobora gukoreshwa nka anticoagulants muri plasma / amaraso yose.Menya ako kanya nyuma yo gukusanya amaraso.
3.Serum na plasma byintangarugero birashobora kubikwa kuri 2-8 ℃ muminsi 3 mbere yo gusuzuma.Niba kwipimisha bitinze kurenza iminsi 3, icyitegererezo kigomba gukonjeshwa (-20 ℃ cyangwa ubukonje).Subiramo gukonjesha no gukonjesha bitarenze inshuro 3.Amaraso yose hamwe na anticoagulant arashobora kubikwa kuri 2-8 ℃ muminsi 3, kandi ntagomba gukonjeshwa;icyitegererezo cyamaraso yose idafite anticoagulant igomba guhita ikoreshwa (niba icyitegererezo gifite agglutination, gishobora gutahurwa na serumu).

UBURYO BUGERAGEZA
Amabwiriza agomba gusomwa rwose mbere yo gukora ikizamini.Emera igenzura ryibikoresho bigereranya ubushyuhe bwicyumba muminota 30 (20 ℃ -30 ℃) mbere yo kwipimisha.Ntukingure ipaki yimbere kugeza yiteguye, igomba gukoreshwa mumasaha imwe iyo ifunguye (Ubushuhe≤60%, Ubushyuhe: 20 ℃ -30 ℃).Nyamuneka koresha ako kanya mugihe ubuhehere ＞ 60%.
Kuri Serumu / Plasma
1. Kuraho igikoresho cyipimisha mumufuka ufunze, ubishyire hejuru yisuku kandi iringaniye hamwe nicyitegererezo hejuru.
2. Ongeramo igitonyanga kimwe (1) cyuzuye cya serumu cyangwa plasma (10μl) uhagaritse murugero rwiza rwa IgM na IgG ukwe.
3. Ongeramo ibitonyanga bibiri (2) (80-100μl) bya sample ya buffer muri sample nziza ya IgM na IgG ukwayo.
4. Itegereze ibisubizo by'ibizamini ako kanya mu minota 15 ~ 20, ibisubizo ntabwo byemewe muminota 20

COVID-19 IgG
Isesengura ryikigereranyo cya COVID-19 IgG Ab igeragezwa ryihuse hamwe na acide nucleic reagent mubisubizo bya serumu:
Igipimo cyiza cyo guhurirana = 46 / (46 + 4) × 100% = 92%,
Igipimo kibi cyo guhura = 291 / (9 + 291) × 100% = 97%,
Igipimo rusange cy'impanuka = ​​(46 + 291) / (46 + 4 + 9 + 291) × 100% = 96.3%.

COVID-19 IgM
Isesengura ryikigereranyo cya COVID-19 IgM Ab kwipimisha byihuse na aside nucleic
reagent muri serumu ntangarugero:
Igipimo cyiza cyo guhurirana = 41 / (41 + 9) × 100% = 82%,
Igipimo kibi cyo guhura = 282 / (18 + 282) × 100% = 94%,
Igipimo rusange cy'impanuka = ​​(41 + 282) / (41 + 9 + 18 + 282) × 100% = 92.3%

ICYITONDERWA
1. Kuri IN VITRO kwisuzumisha gusa.
2. Reagents igomba gukoreshwa vuba bishoboka nyuma yo gufungura.Iyi reagent ntishobora gukoreshwa kugirango ikoreshwe.
3. Igikoresho cyo kwipimisha kigomba kuguma mumifuka ifunze kugeza ikoreshejwe.Niba ikibazo cyo gufunga kibaye, ntugerageze.Ntukoreshe nyuma yitariki yo kurangiriraho.
4.Ingero zose hamwe na reagent bigomba gufatwa nkibishobora guteza akaga kandi bigakorwa muburyo bumwe na virusi yanduye nyuma yo kuyikoresha.