Ibishya Coronavirus COVID-19 IgG / IgM Gusuzuma Byihuse

Ibisobanuro Byihuse

COVID-19 Anti-2020-nCoV Coronavirus Nshya
ibikoresho byo gupima coronavirus COVID-19 igeragezwa ryihuse IgM / IgG ikizamini TUV
Ibishya Coronavirus COVID-19 IgG / IgM Gusuzuma Byihuse

Gupakira & Gutanga

Ibisobanuro

Agashya Coronavirus COVID-19 IgG / IgM Gusuzuma Byihuse AMRPA69
IHame

COVID-19 IgG / IgM Igikoresho cyihuta cyipimisha (Amaraso Yuzuye / Serumu / Plasma) nigitekerezo cyo gukingira immunochromatographic assay.Uwiteka
ikizamini gikoresha antibody anti-muntu IgM (umurongo wikizamini IgM), anti-muntu IgG (umurongo wikizamini IgG) nihene irwanya urukwavu IgG (umurongo ugenzura C)
ubudahangarwa kumurongo wa nitrocellulose.Burgundy ifite amabara ya conjugate arimo zahabu ya colloidal yahujwe na
recombinant COVID-19 antigens yahujwe na zahabu ya colloid (COVID-19 conjugate) hamwe ninkwavu IgG-zahabu.
Mugihe icyitegererezo gikurikirwa na buffer buffer yongeweho kurugero rwiza, IgM & / cyangwa antibodies ya IgG niba ihari, izahuza
COVID-19 conjugate ikora antigen antibodies igoye.Uru ruganda rwimuka binyuze muri nitrocellulose membrane by
ibikorwa bya capillary.Iyo complexe ihuye numurongo wa antibody ihuye (anti-muntu IgM & / cyangwa
anit-muntu IgG) urwego rufashwe rukora ibara ryamabara ya burgundy yemeza ibisubizo byikizamini.Kubura kwa
ibara ryamabara mukarere kizamini ryerekana ibisubizo bidakorwa.
Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy yihene ikingira
kurwanya urukwavu IgG / urukwavu IgG-zahabu conjugate utitaye ku iterambere ryamabara kuri buri tsinda ryibizamini.Bitabaye ibyo, ikizamini
ibisubizo bitemewe kandi icyitegererezo kigomba gusubirwamo hamwe nibindi bikoresho.

UBURYO BWO GUKORA IKIZAMINI
Kuri Serumu cyangwa Plasma Ibigereranyo:
Emerera ibikoresho byo gupima, urugero, buffer na / cyangwa kugenzura kugirango uburinganire bwubushyuhe bwicyumba (15-30 ° C) mbere yo kwipimisha.
1. Kuraho ikizamini cya test / cassette mumifuka ifunze kandi uyikoreshe vuba bishoboka.Ibisubizo byiza bizaboneka niba
isuzuma rikorwa mu isaha imwe.
2. Shira ibikoresho byo kwipimisha hejuru kandi isukuye.
Strip: Ongeramo 2uL ya serumu / plasma kurugero rwicyitegererezo (ahantu h'umuyugubwe hamwe na zahabu ya Colloidal) yumurongo wikizamini, hanyuma wongereho ibitonyanga 2
(hafi 60 μL) ya sample ya buffer kuri paje (hejuru yumurongo) ako kanya.

Cassette:
Ongeramo 2uL ya serumu / plasma kurugero rwiza (A) rwa cassette yikizamini, hanyuma ongeramo ibitonyanga 2 (hafi 60 μL) byicyitegererezo kuri
buffer neza (B) ako kanya.
3. Tegereza umurongo (amabara) ugaragara.Ibisubizo bigomba gusomwa muminota 10.Ibisubizo byiza birashobora kugaragara vuba
nk'iminota 2.Ntugasobanure ibisubizo nyuma yiminota 15