Novel Coronavirus COVID-19 IgG/IgM Diagnostic Degdeg ah

Faahfaahin Deg Deg ah

COVID-19 Ka-hortagga 2020-nCoV Coronavirus cusub
Qalabka baarista coronavirus ee COVID-19 xirmada tijaabada degdega ah ee IgM/IgG TUV
Novel Coronavirus COVID-19 IgG/IgM Diagnostic Degdeg ah

Baakadaha & Bixinta

Tilmaamaha

AMRPA68
Qalabka Tijaabada Degdegga ah ee Ka-hortagga Cudurka COVID-19 IgM/IgG
(Immunochromatography)
MAGACA PRODUCT
Qalabka Tijaabada Degdegga ah ee Ka-hortagga Cudurka COVID-19 IgM/IgG
(Immunochromatography)
ISTICMAALKA KULA JIRA
Reagent-ka waxaa loo isticmaalaa in lagu ogaado fayraska Corona-19 IgM/IgG Antibody gudaha
serum/plasma/dhiiga oo dhan si tayo leh.
MABADA IMTIXAANKA
Xirmadaan waxay ku saleysan tahay mabda'a calaamadda dahabiga ah ee tijaabada immunochromatographic waxayna isticmaashaa habka qabashada si loo ogaado kahortaga COVID-19 IgM/IgG ee muunada.

MABADA IMTIXAANKA

COVID-19 IgM
Marka muunadku ka kooban yahay COVID-19 IgM antibody, waxay samaysaa kakan oo leh calaamadda dahabka antigen (COVID-19 recombinant antigen).Kakan ayaa hore ugu socdaa ficilka chromatography wuxuuna isku daraa antibody-ka dahaarka leh (Mouse anti-human IgM monoclonal antibody) ee khadka T si uu u sameeyo mid adag oo uu yeesho midab (T line), taas oo ah natiijo togan.Marka muunadku aanu ku jirin COVID-19 IgM antibody, wax kakan laguma samayn karo xariiqda T, oo aanay ka muuqan fadh cas, taas oo ah natiijo taban.
Iyadoo aan loo eegin in ka hortagga COVID-19 IgM uu ku jiro muunadda, calaamadda dahabiga ah ee ilaalinta tayada ilaalinta antibody (bakayle IgG antibody) waxay ku xidhmi doontaa difaaca dahaarka leh (Riyaha ka hortagga bakaylaha IgG antibody) ee khadka C si loo sameeyo kakan oo horumariyo midabka (C line).

COVID-19 IgG
Marka muunadku ka kooban yahay COVID-19 IgG antibody, waxay samaysaa kakan oo leh calaamadda dahabka antigen (COVID-19 recombinant antigen).Qalabku hore ayuu u socdaa iyadoo la adeegsanayo chromatography wuxuuna isku daraa antibody-ka dahaarka leh (Mouse anti-human IgG monoclonal antibody) ee khadka T si uu u sameeyo mid adag oo uu yeesho midab (T line), taas oo ah natiijo togan.Marka muunadku aanu ku jirin COVID-19 IgG antibody, wax kakan laguma samayn karo khadka T, oo aanay ka muuqan koox guduudan, taas oo ah natiijo taban.
 

Iyadoo aan loo eegin in COVID-19 IgG antibody uu ku jiro muunada, calaamadda dahabiga ah ee ilaalinta tayada ilaalinta antibody (bakayle IgM antibody) waxay ku xidhmi doontaa difaaca dahaarka leh (Riyaha ka hortagga bakaylaha IgG antibody) ee khadka C si loo sameeyo kakan oo horumariyo midabka (line C).

QAABAHA ugu waaweyn
COVID-19 IgM: T-line oo lagu dahaadhay jiirka lidka-hortagga aadanaha ee IgM monoclonal antibody, sumadda dahabka ah ee suufka adag wajiga COVID-19 recombinant antigen, bakayle IgG antibody, C-line lagu dahaadhay riyaha ka hortagga bakayle IgG antibody.

COVID-19 IgG: T-line oo lagu dahaadhay jiirka lidka-hortagga aadanaha ee IgG monoclonal antibody, sumadda dahabka ah ee suufka adag wajiga COVID-19 recombinant antigen, bakayle IgM antibody, C-line lagu dahaadhay riyaha ka hortagga bakayle IgM antibody.Tusaalaha milanka: oo ka kooban 20 mm fosfate xalinta (PBS)

KAYDINTA IYO DHACINTA
Ku kaydi sida baakadeysan ee boorsada xiran ee 4-30 ℃, iska ilaali kulaylka iyo qorraxda, meel qallalan, oo shaqeyneysa 12 bilood.HA BAFAYN.Tallaabooyinka ilaalinta qaarkood waa in la qaado xagaaga kulul iyo jiilaalka qabow si looga fogaado heerkul sare ama baraf dhalaal.Ha furin baakooyinka gudaha ilaa inta aad diyaar tahay, waa in lagu isticmaalo hal saac haddii la furo (huuminimada≤60%, Heerkulka: 20℃-30℃).Fadlan isticmaal isla markiiba marka uu qoyaanku yahay 60%.

Shuruudaha Tusaalaha
1. Reagent-ka waxa loo isticmaali karaa serum, balaasmaha iyo shaybadhka dhiiga oo dhan.
2. Serum/plasma/sambal dhiiga oo dhan waa in lagu soo qaadaa weel nadiif ah oo qalalan.EDTA, sodium citrate, heparin waxaa loo isticmaali karaa daawada xinjirowga lidka ku ah balaasmaha/muunadaha dhiiga oo dhan.Isla markiiba soo bax ka dib markaad dhiig ururiso.
3.Sambalada serum iyo balaasmaha waxaa lagu kaydin karaa 2-8℃ 3 maalmood kahor baaritaanka.Haddii baaritaanka dib loo dhigo in ka badan 3 maalmood, saamiga waa in la qaboojiyaa (-20 ℃ ama qabow).Ku celi qaboojiyaha oo dhalaali wax aan ka badnayn 3 jeer.Shaybaarada dhiiga oo dhan ee leh daawada xinjirowga lidka ku ah waxa lagu kaydin karaa 2-8℃ 3 maalmood, waana in aan la barafaysan;shaybaarada dhiiga oo dhan oo aan lahayn xinjirowga lidka ku ah waa in isla markiiba la isticmaalo (haddii shaybaarku leeyahay agglutination, waxaa lagu ogaan karaa serum) .

HABKA IMTIXAANKA
Tilmaamaha waa in gabi ahaanba la akhriyaa ka hor inta aan imtixaanka la qaadin.U oggolow kontaroolada aaladda tijaabinta inay u dhigmaan heerkulka qolka 30 daqiiqo (20 ℃-30 ℃) kahor baaritaanka.Ha furin baakooyinka gudaha ilaa inta aad diyaar tahay, waa in lagu isticmaalo hal saac haddii la furo (huuminimada≤60%, Heerkulka: 20℃-30℃).Fadlan isticmaal isla markiiba marka uu qoyaanku yahay 60%.
Loogu talagalay Serum/Plasma
1. Qalabka tijaabada ka saar kiishka xidhan, dul saar meel nadiif ah oo siman iyada oo muunadu si fiican u korayso.
2. Ku dar hal (1) dhibco buuxa oo serum ama balasma (10μl) si toosan ugu geli ceelka muunada IgM iyo IgG si gooni ah.
3. Ku dar laba (2) dhibcood (80-100μl) oo muunad kaydiya ceelka muunada IgM iyo IgG si gooni gooni ah.
4. Isla markiiba u fiirso natiijada 15 ~ 20 daqiiqo gudahood, natiijadu waa been abuur in ka badan 20 daqiiqo

COVID-19 IgG
Falanqaynta heerka isku dhaca ee COVID-19 IgG Ab tijaabada degdega ah iyo reagent nucleic acid ee muunadaha serum:
Qiyaasta shil ee togan=46 / (46+4) × 100% = 92%,
Qiyaasta shilka xun=291 / (9+291) × 100% = 97%,
Wadarta heerka isku beegnaanta=(46+291) / (46+4+9+291) × 100% = 96.3%.

COVID-19 IgM
Falanqaynta heerka isku-dhac ee COVID-19 IgM Ab tijaabada degdega ah iyo aashitada nucleic
reagent ee saamiga serum:
Sicirka shil ee togan=41 / (41+9) × 100% = 82%,
Qiyaasta shilka xun=282 / (18+282) × 100% = 94%,
Wadarta heerka isku beegnaanta=(41+282) / (41+9+18+282) × 100% = 92.3%

FIIRO GAAR AH
1. Loogu talagalay IN VITRO isticmaalka ogaanshaha kaliya.
2. Reagents waa in loo isticmaalo sida ugu dhakhsaha badan ka dib marka la furo.Reagent-kan dib looma isticmaali karo meel la tuuri karo.
3. Qalabka tijaabada ah waa inuu ku jiraa boorsooyinka xidhan ilaa inta laga isticmaalayo.Haddii dhibaatada xidhiddu dhacdo, ha tijaabin.Ha isticmaalin ka dib taariikhda dhicitaanka.
4.Dhammaan shaybaarada iyo reagents waa in loo tixgeliyo inay yihiin kuwo khatar ah oo loo maareeyo si la mid ah wakiilka caabuqa isticmaalka ka dib.