Novel Coronavirus COVID-19 IgG/IgM Diagnostic Rapid

Lintlha tse Potlakileng

Coronavirus e ncha ea COVID-19 Anti-2020-nCoV
coronavirus test kit COVID-19 kit e potlakileng ea teko ea IgM/IgG TUV
Novel Coronavirus COVID-19 IgG/IgM Diagnostic Rapid

Packaging & Delivery

Litlhaloso

AMRPA68
COVID-19 IgM/IgG Antibody Rapid Test Kit
(Immunochromatography)
PRODUCT NAME
COVID-19 IgM/IgG Antibody Rapid Test Kit
(Immunochromatography)
TŠEBELETSO TŠEBELETSO
Sehlahisoa se sebelisetsoa ho bona Corona Virus-19 IgM/IgG Antibody ho
serum/plasma/mali kaofela ka boleng.
TEKO MOLAOOA
Setsi sena se ipapisitse le molao-motheo oa tlhahlobo ea khauta ea label immunochromatographic mme se sebelisa mokhoa oa ho ts'oara ho bona lesole la 'mele la COVID-19 IgM/IgG sampoleng.

TEKO MOLAOOA

COVID-19 IgM
Ha sampole e na le anti-thibela-mafu ea COVID-19 IgM, e theha motsoako o nang le letlapa la khauta la antigen (COVID-19 recombinant antigen).E rarahaneng e tsoela pele tlas'a ketso ea chromatography 'me e kopanya le antibody e koahetsoeng (Mouse anti-human IgM monoclonal antibody) moleng oa T ho theha 'mala o rarahaneng le ho ntshetsa pele (T line), e leng sephetho se setle.Ha sampole e sena anti-anti-COVID-19 IgM, ha ho na motsoako o ka etsoang moleng oa T, 'me ha ho na sehlopha se sefubelu se hlahang, e leng sephetho se fosahetseng.
Ho sa tsotelehe hore na antibody ea COVID-19 IgM e teng sampoleng, antibody ea taolo ea boleng ba leibole ea khauta (antibody ea mmutla ea IgG) e tla tlama le antibody e koahetsoeng (anti-mmutla ea IgG) moleng oa C ho theha motsoako le ho nts'etsapele. mmala (C line).

COVID-19 IgG
Ha sampole e na le anti-thibela-mafu ea COVID-19 IgG, e theha motsoako o nang le letlapa la khauta la antigen (COVID-19 recombinant antigen).E rarahaneng e tsoela pele tlas'a ketso ea chromatography 'me e kopanya le antibody e koahetsoeng (Mouse anti-human IgG monoclonal antibody) moleng oa T ho theha 'mala o rarahaneng le ho ntshetsa pele (T line), e leng sephetho se setle.Ha sampole e sena anti-anti-COVID-19 IgG, ha ho ntho e rarahaneng e ka thehoang moleng oa T, 'me ha ho na sehlopha se khubelu se hlahang, e leng sephetho se fosahetseng.
 

Ho sa tsotelehe hore na antibody ea COVID-19 IgG e teng sampoleng, antibody ea taolo ea boleng ba leibole ea khauta (antibody ea mmutla ea IgM) e tla tlama le antibody e koahetsoeng (anti-mmutla ea IgG) moleng oa C ho theha motsoako le ho nts'etsapele. mmala (C line).

LIEKETSENG MAHLOKO
COVID-19 IgM: T-line e koahetsoeng ka toeba e thibelang motho IgM monoclonal antibody, letlapa la khauta le tiileng la COVID-19 recombinant antigen, antigen ea mmutla ea IgG, C-line e koahetsoeng ka anti-mmutla ea IgG.

COVID-19 IgG: T-line e koahetsoeng ka toeba e thibelang motho IgG monoclonal antibody, letlapa la khauta le tiileng la COVID-19 recombinant antigen, antigen ea mmutla ea IgM, C-line e koahetsoeng ka anti-mmutla ea IgM.Sample dilution: e entsoe ka 20 mM phosphate buffer solution (PBS)

POLOKO LE QETELA
Boloka joalo ka ha e phuthetsoe ka mokotleng o tiisitsoeng ka 4-30 ℃, qoba mocheso le khanya ea letsatsi, sebaka se omileng, se sebetsang bakeng sa likhoeli tse 12.U SE KE UA QETELA.Mehato e meng ea tšireletso e lokela ho nkoa lehlabula le chesang le mariha a batang ho qoba mocheso o phahameng kapa leqhoa le qhibilihang.U se ke ua bula sephutheloana se ka hare ho fihlela se lokile, se tlameha ho sebelisoa ka hora e le 'ngoe haeba se butsoe (Mongobo≤60%, Temp: 20℃-30℃).Ka kopo sebelisa hang-hang ha mongobo o feta 60%.

TLHOKOMELO MOHLALA
1. Reagent e ka sebelisoa bakeng sa serum, plasma le disampole tsa mali kaofela.
2. Serum / plasma / sampole eohle ea mali e tlameha ho bokelloa ka sejaneng se hloekileng le se omileng.EDTA, sodium citrate, heparin e ka sebelisoa e le li-anticoagulants ho lisampole tsa plasma / mali kaofela.Fumana hang ka mor'a ho bokella mali.
3.Serum le plasma disampole li ka bolokoa ho 2-8 ℃ matsatsi a 3 pele ho tlhahlobo.Haeba tlhahlobo e liehile ho feta matsatsi a 3, sampole e lokela ho hoamisoa (-20 ℃ kapa ho bata).Pheta leqhoa 'me u qhibilihe ka makhetlo a sa feteng 3.Mehlala eohle ea mali e nang le anticoagulant e ka bolokoa ho 2-8 ℃ matsatsi a 3, 'me ha ea lokela ho hoamisoa;disampole tse feletseng tsa mali ntle le anticoagulant li lokela ho sebelisoa hang-hang (haeba sampuli e na le agglutination, e ka fumanoa ka serum).

LIEKETSENG MEKHOA
Litaelo li tlameha ho baloa ka botlalo pele ho etsoa tlhahlobo.Lumella litaolo tsa lisebelisoa tsa liteko hore li lekane le mocheso oa kamore metsotso e 30 (20℃-30℃) pele ho tlhahlobo.U se ke ua bula sephutheloana se ka hare ho fihlela se lokile, se tlameha ho sebelisoa ka hora e le 'ngoe haeba se butsoe (Mongobo≤60%, Temp: 20℃-30℃).Ka kopo sebelisa hang-hang ha mongobo o feta 60%.
Bakeng sa Serum/Plasma
1. Tlosa sesebelisoa sa teko ka mokotleng o koetsoeng, o se behe sebakeng se hloekileng le se lekaneng 'me sampole e phalle hantle.
2. Kenya lerotholi le le leng (1) le feletseng la serum kapa plasma (10μl) ka ho toba ka har'a seliba sa IgM le IgG ka thoko.
3. Kenya marotholi a mabeli (2) (80-100μl) a sampuli ea buffer ka har'a seliba sa IgM le IgG ka thoko.
4. Shebella liphetho tsa liteko hang-hang nakong ea metsotso e 15-20, sephetho se fosahetse ho feta metsotso e 20.

COVID-19 IgG
Tlhahlobo ea sekhahla sa iketsahalletseng sa COVID-19 IgG Ab teko e potlakileng le nucleic acid reagent ka lisampole tsa serum:
Sekhahla se setle sa tsietsi = 46 / (46+4) × 100% = 92%,
Sekhahla se fosahetseng sa iketsahalletseng = 291 / (9+291) × 100% = 97%,
Kakaretso ea sekhahla = (46+291) / (46+4+9+291) × 100% = 96.3%.

COVID-19 IgM
Tlhahlobo ea sekhahla sa iketsahalletseng tsa COVID-19 IgM Ab teko e potlakileng le nucleic acid
reagent ka lisampole tsa serum:
Sekhahla se setle sa liketsahalo tse iketsahalletseng = 41 / (41+9) × 100% = 82%,
Sekhahla se fosahetseng sa liketsahalo tse etsahetseng ka tšohanyetso =282 / (18+282) × 100% = 94%,
Kakaretso ea sekhahla = (41+282) / (41+9+18+282) × 100% = 92.3%

TLHOKOMELO
1. Bakeng sa tšebeliso ea tlhahlobo ea IN VITRO feela.
2. Li-reagents li lokela ho sebelisoa kapele kamoo ho ka khonehang ka mor'a hore li buloe.Reagent ena e ke ke ea sebelisoa hape bakeng sa ho lahloa.
3. Sesebelisoa sa teko se lokela ho lula ka mekotleng e tiisitsoeng ho fihlela se sebelisoa.Haeba bothata ba ho tiisa bo etsahala, u se ke ua etsa liteko.Se sebelise kamora letsatsi la ho felloa ke nako.
4.Lits'oants'o tsohle le li-reagents li lokela ho nkoa li le kotsi 'me li sebetsoe ka mokhoa o ts'oanang le moemeli ea tšoaetsanoang ka mor'a tšebeliso.