Ngokusekelwe kumgaqo wokubophelela okukhuphisanayo
I-lateral flow chromatographic immunoassay
I-50ng / mL i-cut-off concentrations
Eyona Cassette ye-THC eKhawulezayo yoVavanyo lwe-AMRDT112
[OKUHLOSIWEYO UKUSETYENZISWA]
I-Marijuana (THC) iCassette ye-Urine Rapid Test Cassette i-AMRDT112 yi-lateral flow chromatographic immunoassay yokufumanisa umgangatho we-11-nor-∆9-THC-9-COOH kumchamo kwi-cut-off concentrations ye-50ng / mL.
Olu vavanyo lubonelela kuphela ngesiphumo sovavanyo lokuqala.Kufuneka kusetyenziswe enye indlela yeekhemikhali ezizezinye ngakumbi ukuze kufunyanwe iziphumo zohlalutyo eziqinisekisiweyo.Ichromatography yegesi/i-mass spectrometry (GC/MS) yeyona ndlela ithandwayo yokuqinisekisa.Uqwalaselo lwezonyango kunye nesigwebo sobuchule kufuneka sisetyenziswe kuso nasiphi na isiphumo sovavanyo lokusetyenziswa kakubi kwechiza, ngakumbi xa kusetyenziswa iziphumo zokuqala eziqinisekileyo.
[USHWANKATHELO]
I-THC yeyona nto iphambili esebenzayo kwi-cannabinoids (intsangu).Xa utshaya okanye ulawulwe ngomlomo, uvelisa iziphumo ze-euphoric.Abasebenzisi baphazamise imemori yexesha elifutshane kunye nokufunda okucothayo.Basenokufumana iziqendu ezidlulayo zokubhideka kunye nexhala.
Ukusetyenziswa kwexesha elide kakhulu kunokunxulunyaniswa nokuphazamiseka kokuziphatha.Impembelelo ephezulu yokutshaya intsangu kwenzeka kwimizuzu engama-20 ukuya kwengama-30 kwaye ubude bexesha yimizuzu engama-90-120 emva kwecuba elinye.Amanqanaba aphezulu e-metabolites ye-urinary afunyenwe kwiiyure zokuvezwa kwaye ahlala ebonakala kwiintsuku ze-3-10 emva kokutshaya.
Uvavanyo olukhawulezileyo lwe-THC lwe-Urine ye-AMRDT112 luvelisa umphumo omuhle xa ukuxinwa kwe-11-nor-∆9-THC-9-COOH kumchamo kudlula i-50ng / mL.Le yindlela ecetywayo yokunqunyulwa kovavanyo lwemizekelo emihle emiselwe nguLawulo lokuSetyenziswa gwenxa kweZiyobisi kunye noLawulo lweeNkonzo zeMpilo yeNgqondo (SAMHSA, USA).
[UMGAQO]
I-THC Urine Rapid Test AMRDT112 yi-immunoassay esekelwe kumgaqo wokubopha ukhuphiswano.Amachiza anokuthi abekhona kumzekelo womchamo akhuphisana ngokuchasene ne-conjugate yawo yechiza ngokwahlukeneyo kwiindawo ezibophelelayo kwi-antibody yazo ethile.
Ngexesha lovavanyo, i-specimen yomchamo ifudukela phezulu ngesenzo se-capillary.Ichiza, ukuba likhona kumzekelo womchamo ongaphantsi koxinaniso lwalo olusikiweyo, aliyi kuzalisa iisayithi ezibophelelayo ze-antibody ethile.I-antibody iya kuthi emva koko isabele nge-conjugate ye-drug-protein kunye nomgca ombala obonakalayo uya kubonakala kwindawo yovavanyo lwekhasethi yechiza elithile.
Ubukho bechiza ngaphezulu koxinaniso olusikiweyo buya kugcwala zonke iisayithi ezibophelelayo ze-antibody.Ngoko ke, umgca onemibala awuyi kubumba kwingingqi yovavanyo.
Umzekeliso womchamo oneziyobisi awusayi kuvelisa umgca onemibala kwindawo ethile yovavanyo lwekhasethi ngenxa yokhuphiswano lwamachiza, ngelixa umboniso womchamo ongenalo ichiza uya kuvelisa umgca kwindawo yovavanyo ngenxa yokungabikho kokhuphiswano ngamachiza.
Ukuze usebenze njengolawulo lwenkqubo, umgca onemibala uya kuhlala ubonakala kwingingqi yolawulo, ebonisa ukuba umthamo ofanelekileyo we-specimen wongeziwe kwaye i-membrane wicking yenzeke.