I-AMRDT012 Ikhasethi yoVavanyo oluKhawulezayo lweSifo sePhepha

Iinkcukacha eziKhawulezayo

1. Ukukhawuleza.

2. Uvakalelo oluphezulu kunye neenkcukacha.

3. Kulula ukuyisebenzisa.

4. Ichanekile kwaye inokuthenjwa.

5. Ugcino lwe-ambient.

6. I-IgG, i-IgM kunye ne-IgA inokufunyanwa.

Ukupakishwa kunye nokuhanjiswa

Iinkcukacha

I-AMRDT012 Ikhasethi yoVavanyo oluKhawulezayo lweSifo sePhepha

Uvavanyo olukhawulezileyo lokufunyaniswa komgangatho we-anti-TB antibodies (Isotypes IgG, IgM kunye ne-IgA) kwigazi elipheleleyo, i-serum okanye isampuli zeplasma.

Ukusetyenziswa koxilongo lwe-in vitro kuphela.

【UKUSETYENZISWA OKUJOLISWEYO】

Ikhasethi yoVavanyo oluKhawulezayo lweSifo sePhepha (igazi elipheleleyo/iSerum/i-Plasma) yikhromatografi ekhawulezayo.

i-immunoassay yokufunyaniswa komgangatho we-anti-TB antibodies (Isotypes IgG, IgM kunye ne-IgA) ngokupheleleyo

igazi, i-serum okanye iisampuli zeplasma.

I-AMRDT012 Ikhasethi yoVavanyo oluKhawulezayo lweSifo sePhepha

1. Ukukhawuleza.

2. Uvakalelo oluphezulu kunye neenkcukacha.

3. Kulula ukuyisebenzisa.

4. Ichanekile kwaye inokuthenjwa.

5. Ugcino lwe-ambient.

6. I-IgG, i-IgM kunye ne-IgA inokufunyanwa.

I-AMRDT012 Ikhasethi yoVavanyo oluKhawulezayo lweSifo sePhepha

【UMGAQO】

Ikhasethi yoVavanyo oluKhawulezayo lweSifo sePhepha (iGazi Elipheleleyo/iSerum/iPlasma) sisigaba esisemgangathweni, esiqinileyo,

Isandwich yesangweji ye-immunoassay eneendawo ezibini zokubona izilwa-buhlungu ezichasene ne-TB kwigazi lonke, kwiserum okanye

iisampuli zeplasma.I-membrane igqunywe kwangaphambili nge-antigen ye-TB kwindawo yovavanyo

yeKhasethi.Ngexesha lovavanyo, izilwa-buhlungu ezilwa ne-TB, ukuba zikhona kwigazi elipheleleyo, kwiserum okanye kwiplasma

Isampulu sisabela kunye namasuntswana agqunywe nge-antigen ye-TB.Umxube ufudukela phezulu

kwi-membrane ngokwechromatographical nge-capillary action ukusabela ne-TB recombinant antigen

i-membrane kwaye ivelise umgca onemibala.Ubukho balo mgca onemibala kwindawo yokuvavanya kubonisa a

isiphumo esihle, ngelixa ukungabikho kwayo kubonisa isiphumo esibi.Ukusebenza njengolawulo lwenkqubo, a

umgca onemibala uya kuhlala ubonakala kummandla womgca wolawulo obonisa ukuba umthamo ofanelekileyo wesampuli

yongeziwe kwaye i-membrane wicking yenzekile.

【IMIQATHANGO】Ukusetyenziswa koxilongo lwe-in vitro kuphela.Musa ukusebenzisa emva kokuphelelwa kwexesha.Ungatyi, usele okanye utshaye kwindawo apho imizekelo okanye iikiti ziphathwayo.Ungasebenzisi uvavanyo ukuba ipakethe yonakele.Yiphathe yonke imizekelo ngokungathi ineentsholongwane ezosulelayo.Qwalasela izilumkiso ezisekelwe kwiingozi zebhayoloji ngexesha lonke lovavanyo kwaye ulandele iinkqubo ezisemgangathweni zokulahlwa ngokufanelekileyo kwemizekelo.Nxiba iimpahla ezikhuselayo ezinjengeedyasi zaselabhoratri, iiglavu ezilahlwayo kunye nokhuseleko lwamehlo xa kuvavanywa imizekelo. ilahlwe ngokwemigaqo yendawo.Musa ukusebenzisa i-potassium oxalate njenge-anticoagulant ukuqokelela i-plasma okanye iisampulu zegazi le-venous.