Ikhasethi elingcono kakhulu le-THC Lokuhlola Okusheshayo AMRDT112

Imininingwane Esheshayo

Ngokusekelwe kumgomo wokubopha ngokuncintisana

I-lateral flow chromatographic immunoassay

50ng/mL ukugxilisa okusikiwe

Ukupakisha Nokulethwa

Imininingwane

Ikhasethi elingcono kakhulu le-THC Lokuhlola Okusheshayo AMRDT112

[UKUSEBENZISA OKUHLOSIWE]
I-Marijuana (THC) Ikhasethi Lokuhlola Okusheshayo Komchamo i-AMRDT112 iyi-assay ye-lateral flow chromatographic yokutholwa kwekhwalithi ye-11-nor-∆9-THC-9-COOH emchameni ekugxiliseni okunqanyuliwe okungu-50ng/mL.

Lesi sivivinyo sinikeza umphumela wokuqala wokuhlaziya kuphela.Enye indlela yamakhemikhali ecacile kufanele isetshenziswe ukuze kutholwe umphumela wokuhlaziya oqinisekisiwe.I-Gas chromatography/mass spectrometry (GC/MS) iyindlela yokuqinisekisa ekhethwayo.Ukucatshangelwa komtholampilo kanye nokwahlulela kochwepheshe kufanele kusetshenziswe kunoma yimuphi umphumela wokuhlolwa kokuhlukumeza, ikakhulukazi uma kusetshenziswa imiphumela yokuqala emihle.

[ISIFINYEZO]
I-THC iyisithako esiyinhloko esisebenzayo kuma-cannabinoids (insangu).Uma ubhenywa noma uphuzwa ngomlomo, ukhiqiza imiphumela ejabulisayo.Abasebenzisi bakhubazeke inkumbulo yesikhashana futhi behlisa ukufunda.Bangase futhi babe neziqephu zesikhashana zokudideka nokukhathazeka.
Ukusetshenziswa isikhathi eside okunzima kakhulu kungase kuhlotshaniswe nokuphazamiseka kokuziphatha.Umphumela omkhulu wokubhema insangu uvela emizuzwini engama-20-30 kanti ubude besikhathi yimizuzu engama-90-120 ngemuva kogwayi owodwa.Amazinga aphakeme ama-metabolites omchamo atholakala emahoreni okuchayeka futhi ahlala ebonakala izinsuku ezi-3-10 ngemuva kokubhema.

I-THC Urine Rapid Test AMRDT112 iveza umphumela omuhle lapho ukugxila kwe-11-nor-∆9-THC-9-COOH emchameni kudlula 50ng/mL.Lesi isiphakamiso sokunqanyulwa kokuhlolwa kwezibonelo ezinhle ezibekwe yi-Abuse Abuse and Mental Health Services Administration (SAMHSA, USA).

[ISIMISO]
I-THC Urine Rapid Test AMRDT112 iwukuhlolwa kokuzivikela komzimba okusekelwe kumgomo wokubopha okuncintisanayo.Izidakamizwa okungenzeka zibe khona esibonelweni somchamo ziqhudelana nenhlanganisela yazo yezidakamizwa ukuze zibophe amasayithi ku-antibody yazo ethile.

Ngesikhathi sokuhlolwa, isampula yomchamo ihambela phezulu ngesenzo se-capillary.Umuthi, uma ukhona esifanekisweni somchamo ngaphansi kokugxila kwawo esikiwe, ngeke ugcwalise amasayithi okubopha ama-antibody awo athile.I-antibody izobe isisabela nge-conjugate yesidakamizwa-protein futhi umugqa onemibala ebonakalayo uzovela esifundeni somugqa wokuhlola wekhasethi lesidakamizwa esithile.

Ukuba khona komuthi ngaphezu kokugxiliswa okunqunyiwe kuzogcwalisa zonke izingosi ezibophayo ze-antibody.Ngakho-ke, umugqa onemibala ngeke wakhe endaweni yomugqa wokuhlola.
Isampula somchamo onomuthi ngeke sikhiqize umugqa onemibala endaweni ethile yokuhlola yekhasethi ngenxa yokuncintisana kwezidakamizwa, kuyilapho isifanekiso somchamo ongenawo umuthi sizokhiqiza umugqa endaweni yokuhlolwa ngenxa yokungabikho kokuncintisana kwezidakamizwa.

Ukuze usebenze njengesilawuli senqubo, umugqa onemibala uzohlala uvela endaweni yomugqa wokulawula, okubonisa ukuthi umthamo ofanele we-specimen wengeziwe futhi i-membrane wicking yenzekile.