Ikhwalithi ephezulu ye-AM3705 yokuhlaziya isidoda esizenzakalelayo

Imininingwane Esheshayo

• Ingakwazi ukuhlunga zonke izinhlobo zokungcola okwehlukile kusidoda ukuze kuqinisekiswe ukunemba kokuhlolwa.
• Ukuhlolwa kwe-Scatheless kungaqinisekisa ukunyakaza kwemvelo kwesidoda kanye nesampula ye-monolayer.
• Yonke imininingwane nezithombe eziguquguqukayo nezimile zokuhlolwa kwesidoda ezigulini zigcinwa ngokwedijithali.Ingasetshenziselwa ukubuza okuhlukahlukene, ukulungisa, ukuhlanganisa, kanye nemibiko yokuhlola yokuphrinta, futhi kwabelwane ngayo ngaphakathi kwenethiwekhi ngokukhululekile.
• Isofthiwe ethuthukisiwe yokuhlaziya i-morphological, isithombe esinemibala ecacile futhi enembile.

Ukupakisha Nokulethwa

Imininingwane

1. Isingeniso se-Apparatus

Umkhiqizo okhaliphile osetshenziswa emithini yesimanje yokuzala nenhlanganisela yobuchwepheshe bokucubungula izithombe zekhompyutha kanye nobuchwepheshe bobuhlakani bokwenziwa obusezingeni eliphezulu.Ingasetshenziselwa ukuhlonza isidoda ngokuzenzakalelayo kanye nokulandelela okuzenzakalelayo kokunyakaza kwesidoda ngokushesha, iqedele ukuhlaziya imingcele eminingana ngokuhambisana namazinga omhlaba e-WHO futhi ithathe ukuhlaziya okubanzi komthamo womsebenzi wesidoda nezici ezimile.Ilungele ukuhlolwa kwesidoda emtholampilo, ihlinzeka ngesisekelo esibalulekile sesayensi sokuzala kwabesilisa.

2. Izici ze-Apparatus

1) Ingakwazi ukuhlunga zonke izinhlobo zokungcola okuhlukile kwesidoda ukuze kuqinisekiswe ukunemba kokuhlolwa.2) Ukuhlolwa kwe-scatheless kungaqinisekisa isimo sokunyakaza kwemvelo kwesidoda kanye nesampula ye-monolayer.3) Yonke idatha nezithombe ezishukumisayo nezimile zokuhlolwa kwesidoda ezigulini. agcinwe ngokwedijithali.Ingasetshenziselwa ukubuza, ukulungisa, ukuhlanganisa, kanye nemibiko yokuhlola yokuphrinta, futhi kwabelwane ngayo ngaphakathi kwenethiwekhi ngokukhululeka.4) Isofthiwe yokuhlaziya yokwakheka kwemvelo ethuthukisiwe, isithombe esinemibala ecacile nenembile.

3. Izinto Zokuhlola

1) Ukuminyana kwesidoda, izinga lokusinda kwezidoda, ukunyakaza kwendawo yesidoda, kanye nejika lokusatshalaliswa kokunyakaza kwesidoda2) Isivinini esimaphakathi, inani eliphelele lama-sperms, inani eliphelele lama-sperms anyakazayo, izinga lokusinda lama-sperms ekuhambeni kwe-curvilinear ama-sperms3) Isivinini esimaphakathi, inani eliphelele lama-sperm, inani eliphelele lama-sperms anyakazayo, izinga lokusinda kwezidoda ekuhambeni kwe-rectilinear yesidoda4) Isivinini esimaphakathi, inani eliphelele lama-sperms, inani eliphelele lama-sperms anyakazayo, izinga lokusinda kwezidoda. ekuhambeni kwe-avareji yama-sperms5) Isivinini esenziwe ngezigaba sokunyakaza kwesidoda: izinga lokusinda lika-A ukunyakaza ngokushesha, B ukuya phambili kancane, C ukungahambi ukuya phambili, D hyperslow noma ukunganyakazi kwezidoda6) I-amplitude eseceleni yesidoda, Uphiko lwesidoda, imvamisa yokushaywa kwezidoda, izinga lokunyakaza kwe-rectilinear, inombolo ephelele7) Isivinini esilinganayo, isivinini esimaphakathi sokunyakaza, inombolo yesidoda yokunyakaza okulandelanayo

4. Imingcele Yezobuchwepheshe

1) Ubukhulu bezidoda ezihloliwe: 10002) Ibanga lesivinini sokuhlola: 0-180um/s3) Inombolo yozimele yesithombe: 0-754) Ukulungiswa kobubanzi bezinhlayiyana: 0-150µm/s5) Isikhathi sokuhlaziya: 1-5 amasekhondi noma ngaphezulu6 ) Inombolo yeqembu yezithombe eziqoqiwe: 1-15 amaqembu7) Ilensi yomgomo weMicroscope: 10x.20x.25x.40×8) Okuqukethwe okubonisiwe kwesistimu yokuhlola isidoda akukho ngaphansi: 1) ijika lokusabalalisa lezidoda ezimile, 2) idatha yezici yesidoda kanye nedatha yezibalo ekuhlaziyweni kwayo yonke imisebenzi esemqoka, 3) ijika le-sperm dynamic trajectory, 4) bonisa isivinini sokunyakaza kwezidoda ezihlukahlukene futhi amandla kuyimephu ehambisanayo yezidoda, 5) ulwazi lwecala lokuphatha njengegama leziguli;9) Okukhiphayo okuqukethwe okuvela ohlelweni lokuhlola izidoda akukho ngaphansi: 1) idatha yobuchwepheshe eyinhloko yesidoda, 2) ijika le-sperm dynamic trajectory, 3) ukuhlaziywa kanye ne-determinant histogram 4) ulwazi lwecala lokuphatha njengegama leziguli

5. Ukucushwa Okujwayelekile

Iyunithi Eyinhloko, Ikhompyutha, I-LCD Monitor, Iphrinta, I-Microscope, isofthiwe ye-English Analyzer