I-Novel Coronavirus COVID-19 IgG/IgM Diagnostic Rapid

Imininingwane Esheshayo

I-COVID-19 Anti-2020-nCoV ICoronavirus entsha
ikhithi yokuhlola i-coronavirus ye-COVID-19 ikhithi yokuhlola okusheshayo ye-IgM/IgG yokuhlola i-TUV
I-Novel Coronavirus COVID-19 IgG/IgM Diagnostic Rapid

Ukupakisha Nokulethwa

Imininingwane

Inoveli yeCoronavirus COVID-19 IgG/IgM Diagnostic Rapid AMRPA69
ISIMISO

Ikhithi ye-COVID-19 IgG/IgM Rapid Test (Whole Blood/Serum/Plasma) iyisivivinyo se-lateral flow immunochromatographic.I
Ukuhlola kusebenzisa i-anti-human IgM antibody (umugqa wokuhlola we-IgM), i-IgG ephikisana nomuntu (umugqa wokuhlola we-IgG) kanye ne-IgG yembuzi elwa nogwaja (umugqa wokulawula C)
I-immobilized emgqeni we-nitrocellulose.Iphedi ye-conjugate enemibala e-burgundy iqukethe i-colloidal gold conjugated to
i-recombinant COVID-19 antigens ehlanganiswe negolide le-colloid (COVID-19 conjugates) kanye nezihlanganisi zikanogwaja we-IgG-gold.
Uma isifanekiso esilandelwa yibhafa ye-assay yengezwa emthonjeni wesampula, amasosha omzimba e-IgM &/noma e-IgG uma ekhona, azobophezela
I-COVID-19 ihlanganisa ukwenza ama-antigen antibodies abe inkimbinkimbi.Le nkimbinkimbi ifuduka ngolwelwesi lwe-nitrocellulose ngokuthi
umsebenzi we-capillary.Lapho inkimbinkimbi ihlangana nomugqa we-anti-immobilized ehambisanayo (anti-human IgM &/noma
i-anit-human IgG) inkimbinkimbi ibambeke yenza ibhande elinemibala e-burgundy eliqinisekisa umphumela wokuhlolwa osebenzayo.Ukungabibikho kwe
ibhendi enemibala endaweni yokuhlola ibonisa umphumela wokuhlola ongasasebenzi.
Ukuhlola kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinombala obomvana lembuzi eyi-immunocomplex
i-anti rabbit IgG/rabbit IgG-gold conjugate ngaphandle kokuthuthuka kombala kunoma yimaphi amabhendi okuhlola.Uma kungenjalo, ukuhlolwa
umphumela awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.

INQUBO YOKUHLOLA
Ngezibonelo zeSerum noma zePlasma:
Vumela ikhithi yokuhlola, isifanekiso, ibhafa kanye/noma izilawuli ukuze zilingane nezinga lokushisa legumbi (15-30°C) ngaphambi kokuhlolwa.
1. Khipha umucu wokuhlola/ikhasethi esikhwameni se-foil esivaliwe futhi uyisebenzise ngokushesha ngangokunokwenzeka.Imiphumela emihle kakhulu izotholakala uma
ukuhlolwa kwenziwa phakathi nehora elilodwa.
2. Beka ikhithi yokuhlola endaweni ehlanzekile nesezingeni.
Umdweshu:Faka u-2uL we-serum/plasma kuphedi yesampula(indawo ensomi enegolide eliyi-Colloidal) yomugqa wokuhlola, bese wengeza amaconsi ama-2
(cishe u-60 μL) wesampula sebhafa kuphedi yebhafa (phezulu komugqa) ngokushesha.

Ikhasethi:
Engeza u-2uL we-serum/plasma esibonelweni somthombo(A) wekhasethi lokuhlola, bese wengeza amaconsi ama-2 (cishe ama-60 μL) esampula yebhafa
i-buffer kahle (B) ngokushesha.
3. Linda kuze kuvele i(ama)layini anombala.Umphumela kufanele ufundwe ngemizuzu eyi-10.Imiphumela emihle ingase ibonakale maduze
njengemizuzu emi-2.Ungahumushi umphumela ngemva kwemizuzu engu-15